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Colorimetric and turbidimetric analysis of endotoxins using the absorbance detection mode
Torn between a colorimetric or turbidimetric LAL assay? Whichever path you take, BMG LABTECH’s absorbance readers deliver dependable results every time.
PHERAstar FSX
功能强大，高度灵敏的HTS酶标仪
PHERAstar® FSX是最新的高度灵敏的多功能酶标仪，适用于高通量筛选，并结合高灵敏度与迅捷的读取速度。 基于BMG Labtech独特的光学模块系统、同时双发射、动态范围扩展技术, UV/vis分光计、AAS系统以及专用的AlphaScreen®和TRF激光器，PHERAstar FSX即使在3456孔微孔板中也能轻松快速地进行分析检测。
评分
A must equipment for any lab!
PheraStar is really easy to use, to assess and define protocol in order to get reproducible...
4.8/5.0 (81)
PHERAstar FSX
不再担心滤光片和二向色性的安装：PHERAstar FSX配备了易于操作、经过优化的光学模块，这些模块包含所有应用特定的滤光片，反光镜，二向色性和/或起偏器，并且可以被酶标仪自动识别。 另外，PHERAstar FSX配备了四个优化的光电倍增管（PMT），可自动选择相应的检测模式。 由于有同时双发射测量模式，您还可以同时进行两个发射波长的测量。
动态范围扩展技术和自动对焦技术摈弃了手动设置，检测优化比以往任何时候都更容易
出于高通量药筛自动化目的，PHERAstar FSX可轻松整合到自动化系统中，并支持优化机器人的整合能力和多用户控制。
PHERAstar FSX 仅用于科研用途。
特点
- 在荧光强度和极化方面是最灵敏的酶标仪
- 唯一具有5个检测专用检测器的酶标仪
- 同时双发射光测量，包括Alpha技术
- 动态范围扩展技术和自动对焦
- AlphaScreen®/AlphaLISA®/ AlphaPlex™专用激光光源
- 新一代TRF激光光源
- 顶部和底部焦距调整
- 高精度注射器，同时进行试剂注入和检测
- 高端分析稳定性系统
深受信任和喜爱：
可选配件
- THERMOstar: 微孔板培养箱和轨道式振荡器
- Microplate Stacker: マイクロプレート処理システム
- LVis Plate: 用于低体积测量和性能测试的微孔板
- Optic Modules: 适用于多种应用的滤光片
- Evaluation Plate: 用于光学功能验证的微孔板
PHERAstar FSX
我们的技术
-
PHERAstar FSX 需要哪些维护？
BMG LABTECH 微孔板读数仪坚固可靠，无需对光学组件进行校准。建议的维护包括板托偏移校准、光学部件清洁、软件/固件更新以及例行性能检查。 -
维护费用和计划有哪些？
BMG LABTECH 提供灵活的维护计划以确保长期性能。费用取决于所选计划。请通过服务合同表联系我们。 -
是否提供技术支持或培训？
是的，我们提供全面的技术支持和培训服务，以帮助用户最大化仪器性能。请联系我们了解更多信息。 -
在哪里可以找到 PHERAstar FSX 的更多信息或购买选项？
您可以在 PHERAstar FSX 产品页面找到详细信息、规格及可选配置。若需购买选项、报价或个性化建议，请直接提交销售请求。 -
如何申请额外的光学模块？
可通过我们的过滤器申请表索取报价。 -
在哪里可以获取 PHERAstar FSX 的使用手册？
每台仪器都附带手册。如遗失，请使用手册申请表重新获取。 -
软件在哪里获取？
软件随读数仪附带，可安装在任意数量的电脑上。随附于 USB（或旧型号的光盘）内。如丢失，我们可提供下载链接。请联系软件支持。 -
软件是否兼容 Windows 11？
是的，完全兼容。如需安装帮助或兼容性支持，请联系软件支持。 -
设备购置成本是多少？
根据所选配置而定。请联系我们获取个性化报价。 -
是否有测量优化教程？
是的，我们提供 How-To Notes，解释检测参数及优化技巧。可在 How-To Notes 页面查看。 -
典型交货时间？
取决于型号库存、配置和地区。请在联系页面提交一般咨询。
Measurement of a reconstituted circadian clock using fluorescence polarization
Unlocking Nature’s Timekeepers. Learn how to read the internal clock of organisms and how to decode life’s 24-hour rhythm at the molecular level using a microplate reader.
Multiplexing the production of two G protein-coupled receptor second messengers using spectrally resolved fluorescent dyes
Streamline your workflow and double your insights: multiplexing cellular signal responses of multiple second messengers in a single measurement saves valuable time and conserves reagents.
Detection of bacterial endotoxins using the PYROSTAR™ ES-F/Plate LAL assay
Perform the PYROSTAR ES-F/Plate LAL test your way— BMG LABTECH readers support both turbidimetric and nephelometric evaluation for maximum flexibility.
Determination of Minimum Inhibitory Concentration (MIC) of antibiotics using OD600
Do you want to make your MIC measurements more efficient and automated? Find out here how BMG LABTECH microplate readers can support you in this task.
Stream-lined detection of endotoxin using the ENDOZYME II recombinant factor C assay and Enhanced Dynamic Range on the VANTAstar
Do you want to streamline your endotoxin detection workflow? Check out what the combination of VANTAstar and ENDOZYME II can do for you.
Detection Modes
Fluorescence intensity - incl. FRET
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlex™
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/Vis absorbance spectra
Measurement Modes
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Simultaneous Dual Emission
Spectral scanning (absorbance)
Real-time ratiometric measurements
Well scanning
Microplate Formats
Up to 3456-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier
Robot compatible
Light Sources
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™
Dedicated laser for TRF and TR-FRET
Detectors
Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes
CCD spectrometer
Wavelength Selection
Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application)
For top and bottom reading
UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well
Spectral Range
Filters
230 - 750 nm or 230 - 900 nm for FI, FP
230 - 750 nm for LUM
230 - 900 nm for TRF
Spectrometer
220 - 1000 nm for ABS; wavelength precision: ≤ 0.5 nm
Sensitivity
FI
(top)
< 0.15 pM FITC (384sv black, 20 μL)
< 0.5 pM FITC (1536 black, 8 μL)
Dynamic Range: 8 decades
FI
(bottom)
<1.0 pM FITC (384g black, 50 μL)
FP
< 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
< 1.5 mP SD at 1 nM FITC (1536 black, 8 μL)
TRF with laser.
< 5 fM europium (384, 80 μL)
< 15 fM europium (1536, 8 μL)
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 1100% (High Calibrator)
Delta F > 25% (Low Calibrator)
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
Dynamic Range: 8 decades
AlphaScreen® with laser
< 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)
ABS with spectrometer
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD; photometric resolution: 0.001 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Linearity: ≤ 0.8% at 2.0 OD
Read Times
Flying mode (1 flash)
14 s (384)
27 s (1536)
10 flashes
38 s (384)
1 min 52 s (1536)
50 flashes
1 min 29 s (384)
5 min 18 s (1536)
Shaking
Linear, orbital, and double-orbital with user-definable time and speed
Incubation
+5 °C above ambient up to 45 °C
The upper heating plate of the incubation chamber operates at +0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
With Advanced Assay Stability (AAS) system: from 18 - 45°C
Software
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Dimensions
Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg
With AAS system: width: 48 cm, depth: 68 cm, height: 47 cm; weight: 62 kg
Optical Path Guides
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Z-Adjustment
Automatic focal height adjustment (0.1 mm resolution)
Reagent Injection
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing
Integrated Barcode Reader
Up to three integrated barcode readers
Power Requirements
- 100 or 115 or 230 V, 50/60 Hz, auto ranging
Power Consumption
- Normal operating conditions: 50 W
- Idle State: 20 W
- AAS-option: + 30 W
- Switched off: 0 W max. 300 VA
Optional Accessories
LVis Plate
Sample Capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates
THERMOstar
Microplate incubator and shaker
Stacker
Magazines for up to 50 plates - continuous loading feature
Upgrades
Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc
*
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates
The PHERAstar FSX is for research use only.